Speed and speci
ficity
for routine and high-throughput PCR
Phire Hot Start DNA Polymerase is
fast, robust and capable o
f ampli
fying long DNA
fragments with high yields. These
features are achieved through advanced protein engineering; the polymerase incorporates a dsDNA-binding domain which allows short extension times (20 s/kb) and improves yields. The unique A
ffibody-based inactivation method o
f Phire Hot Start DNA Polymerase increases the speci
ficity o
f the ampli
fication at ambient temperatures but allows immediate and complete reactivation o
f the enzyme at standard cycling temperatures. The polymerase is ideal
for routine and high-throughput PCR.
See details
Includes:
Phire Hot Start DNA Polymerase is supplied with 5x Phire Reaction Buffer and DMSO. The buffer provides 1.5 mM MgCl2 in the final 1x concentration.
